AMIDD (2021) Lecture 5: Offline activities
Dear participants of the AMIDD 2021 course,

Please find below the form to collect your answers to the questions about the book chapter, Evaluation of the Biological Activity of Compounds: Techniques and Mechanism of Action Studies, by Iain G. Dougall and John Unitt in the book The Practice of Medicinal Chemistry.

The aim of the activity is to prepare you for the material that we will talk about in the next section. In case you have difficulties understanding the material, do not worry - we will address them in the next session as well.

In the upcoming course, I will try to address issues that are particularly confusing, and questions that are challenging or interesting for many of you.

Best regards,
David
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Q1. An important chemical and mathematical concept was not described in the book chapter: what does the Law of Mass Action mean?
Q2: Which quantity measures binding affinity directly: dissociation constant (K_D) or the concentration of the test compound that produces 50 percent inhibition (IC_{50})?
Clear selection
Q3: In Figure 2.3, what do x- and y-axis represent in panel (A) and panel (B), respectively?
Q4: What is a sigmoidal curve?
Q5: Do IC_{50} values indicate a particular mechanism of action (MoA)?
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Q6: In a certain enzymatic assay, two compounds have the following pIC50 values: 7.2 (Compound A), 9.3 (Compound B). If all other conditions are held constant, what is the relationship between binding affinities of the two compounds with regard to the target?
Clear selection
Q7: Why is DMSO often used in bioassays?
Q8: Can you use your own language to describe what is the Hill function?
Q9: What statistical measure is used to measure the signal-noise ratio in screening? Can you use your own language explaining it?
Q10: Why logarithm (usually base 10) transformation is often preferred to represent quantities such as IC_{50} and K_{i}?
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