Biotech Practice Exam: Reagent Preparation & Proper Use of Lab Equipment

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1. A recipe for making LB agar requires that 40 g of agar are added to 1000 ml of distilled water. How much LB agar would be used for make 750 mL of LB agar? *

1 point

20 g

30 g

40 g

50 g

2. The recipe on a bottle of powdered agar says to mix 12 g of powder with every 25 ml of distilled water. How much agar would you need to make 850 ml of agar? *

1 point

1778.3

40.8 g

408.00 g

177.8 g

3. How many grams of agarose powder would you need to prepare 225 ml of a 2% electrophoresis gel? *

1 point

2.0 g

2.5 g

4.50 g

25 g

4. To make 3 Liters of a 2 molar EDTA solution, how many grams of EDTA would need to be added? (C10H16N2O8; Molar mass: 292.24 g/mol) *

1 point

876.72 g

1753.44 g

292.24 g

584.48 g

5. The OD600 of an initial stock of Penicillium spores is 0.84. Calculate the volume of spore solution needed for 25 mL with a final concentration of OD600 0.05. *

1 point

1.49 mL

1.25 mL

1.05 mL

0.84 mL

6. How much Tween 20 (an emulsifier) is needed to prepare 250 mL of 10% Tween 20 solution? *

1 point

2.5 mL

25 mL

10 mL

1.0mL

7. How much BSA is needed to prepare 50 mL of 5 mg/mL of BSA ? *

1 point

2.5 mg

25 mg

250 mg

2500 mg

8. How much 50X TAE buffer is needed to make 1 liter of 1X TAE buffer? *

1 point

5 mL

50 mL

2 mL

20 mL

9. How much NaOH is needed to make 50 mL of 15 mg/mL NaOH from 100 mg/mL NaOH? *

1 point

7.5 mL

75 mL

1.5 mL

15 mL

10. How much NaCl is needed to prepare 400 mL of 5% NaCl? *

1 point

5. g

2 g

50 g

20 g

11. If you are using a solution in lab that has a neutral pH, and you would like to make it slightly acidic. Which of the substances could be added to the solution to accomplish this? *

1 point

NaOH

NaCl

HCl

12. For a step in a lab protocol, you need to pipette 19 uL of a liquid into a tube. Which piece of equipment would do this most accurately? *

1 point

2 uL - 20 uL pipette

0.1 uL - 10 uL pipette

100 uL - 1000 uL pipette

20 uL - 200 uL pipette

13. In a lab, an autoclave is used to *

1 point

Determine the concentration of chemicals or other substances in a solution.

Maintain a sterile environment in which to transfer and plate micro-organisms

Maintain a constant temperature that allows bacterial samples to grow

Sterilize substances and equipment using heat and pressure

14. What is the best way to determine the concentration of bacteria growing in a liquid medium? *

1 point

Extract the DNA from the bacteria and run electrophoresis with it

Measure the optical density of a sample of the solution with a spectrophotometer

Hold a sample of the solution up to examples and estimate

Count cells in a sample under a microscope using a hemocytometer

15. The instrument shown below is called a(n) *

1 point

spatula

spreader

innoculating loop

scoop loop

16. When using PCR reagents have been stored in the freezer, which piece of lab equipment should be used to mix the thawed reagents before using them in a PCR reaction? *

1 point

A vortexer

A centrifuge

An autoclave

A hot plate and stirrer

17. DNA can be stored for years at which of the following temperatures? *

1 point

4 degrees Celsius

-20 degrees Celsius

-80 degrees Celsius

Room temperature

Required

18. DNA samples that have been amplified through PCR can be stored for a few days at *

1 point

4 degrees Celsius

-20 degrees Celsius

-80 degrees Celsius

Room temperature

19. When E. coli has reached stationary growth in LB broth, is can be stored for the short term at: *

1 point

4 degrees Celsius

-20 degrees Celsius

-80 degrees Celsius

Room temperature

20. Restriction enzymes and taq polymerase are always stored at *

1 point

4 degrees Celsius

-20 degrees Celsius

-80 degrees Celsius

Room temperature

21. To store E. coli (or other bacteria) long term, it is best to *

1 point

store the cells in 50% sterile glycerol at -20 degrees Celsius

store the cells in 50% sterile glycerol at -80 degrees Celsius

store the cells in 50% sterile glycerol at 4 degrees Celsius

store it in LB broth at -80 degrees Celsius

22. Prior to autoclaving LB agar, it is important to completely dissolve all solutes (solids) using a *

1 point

vortexor

centrifuge

hot plate and stirrer

gel electrophoresis rig and power supply

23. Prior to working in a Biosafety cabinet or laminar flow hood it is important that *

1 point

the temperature inside the hood is at 37 degrees Celsius

70% ethanol, 10% bleach and 95% ethanol are used to clean all inside surfaces of the hood

the laminar air flow is well established

Both B and C are correct

24. To amplify a small section of targeted DNA using Polymerase Chain Reaction, which piece of lab equipment is used? *

1 point

Biosafety cabinet (aka Laminar flow hood)

Autoclave

Hot water bath

Thermocycler

25. During bacterial transformation, which piece of lab equipment is used to heat shock the E. coli? *

1 point

Biosafety cabinet (aka Laminar flow hood)

Autoclave

Hot water bath

Thermocycler

26. The equipment shown below are used to *

1 point

stir bacterial media during preparation

transfer bacteria from LB broth to LB agar plate

spread bacteria on an agar plate to create a bacterial lawn

z streak bacteria to create single Colony Forming Units (CFUs)

27. When doing microbiology at the lab bench, one should work close to a Bunsen burner (or butane burner) because the burner creates a convection cell of rising hot air that *

1 point

kills all the bacteria in the air

kills all the bacteria on or near the lab bench

heats up the microbes so that they grow faster when plated

reduces the chances of air borne bacteria falling directly on your agar plate

28. The final step of DNA isolation requires that a pellet of DNA be formed by separating the DNA from all the other reagents in the reaction. To separate out the DNA you would use a *

1 point

centrifuge

vortexor

micropipettor

electronic balance

29. When doing microbiology the best way to reduce contamination is to work with samples *

1 point

in a fume hood

in a clean room

in a laminar flow hood

on a sterilized lab bench

30. Any time you use an electronic balance to mass dry reagents, you should always: *

1 point

use a weigh boat to hold the reagents on the balance

tare or zero out the balance using a weigh boat before adding the reagents to the weigh boat

tare or zero out the balance using a flask to hold the reagents on the balance

put the dry reagents directly on the pan of the balance

31. When measuring out 5 mL of sterile LB broth into 15 mL Falcon tubes, which is the best pipette to use? *

1 point

25 mL serological pipette

5 ml serological pipette

p1000 micropipette

25 mL graduated cyclinder

32. To confirm the results of a polymerase chain reaction (PCR), you would use which of the following lab equipment? *

1 point

UV transilluminator

SDS PAGE (Polyacrylamide) gel

agarose gel electrophoresis

a light microscope

33. To separate and visualize proteins, you would use a *

1 point

UV transilluminator

SDS PAGE (Polyacrylamide) gel

agarose gel electrophoresis

a light microscope

34. To visualize DNA you would stain a gel with Ethidium bromide and then look at the results using a *

1 point

UV transilluminator

SDS PAGE (Polyacrylamide) gel

agarose gel electrophoresis

a light microscope

35. Gel Electrophoresis separates molecules based on: *

1 point

Shape and Color

Size and Charge

Shape and Size

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