Biotechnology - Principles and Processes

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Biotechnology - Principles and Processes - 01

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Mrs. Surabhi Mukherjee, (M.Sc., Biotechnology, B.Ed., gurucoolclasseslko@gmail.com)

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Q1. Restriction enzymes were discovered by *

1 point

Smith and Nathans

Alexander Fleming

None

Berg

Q2. Bacteria protect themselves from viruses by fragmenting viral DNA with *

1 point

Exonuclease

Ligase

Endonuclease

Gyrase

Q3. Klenow fragment is derived from *

1 point

DNA Pol-I

Reverse Transcriptase

DNA Ligase

DNA Pol-II

Q4.Southern blotting is *

1 point

Transfer of DNA fragments from electrophoretic gel to a nitrocellulose sheet

Comparison of DNA fragments to two sources

Transfer of DNA fragments to electrophoretic gel from cellulose membrane

Attachment of probes to DNA fragments

Q5. ELISA is *

1 point

Using radiolabelled second antibody

Addition of substrate that is converted into a coloured end product

Usage of RBCs

Using complement-mediated cell lysis

Q6. The Golden Rice variety is rich in *

1 point

Lysine

Biotin

Β-carotene and ferritin

Vitamin C

Q7. The DNA fragments have sticky ends due to *

1 point

Unpaired bases

Calcium ions

Free methylation

Endonuclease

Q8. Plasmids are used as cloning vectors for which of the following reasons? *

1 point

Replicate freely outside bacterial cells

Self-replication in bacterial cells

Can be multiplied in culture

Can be multiplied in laboratories with the help of enzymes

Q9. The human genome project was launched in the year *

1 point

1990

1989

1973

1980

Q10. The vaccines prepared through recombinant DNA technology are *

1 point

First-generation vaccines

Third generation vaccines

Second-generation vaccines

None

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