We kindly invite all PhD students, post-docs and supervisors to participate in Science Day held by KMI (Clinical Medicine) and BSMI (Institutes of Biomedicine and Translational Medicine) at 29-30.11.2019 in Trofee jahimaja. This year our key presenters will be young scientist. Updated information here: http://sime.ut.ee/teaduspaev.

Science Day is excellent way for PhD students to relax and network. In addition to the scientific program we will have an entertaining evening.

For more information or special cases contact us:
teaduspaev@gmail.com

Kind regards,
organizing committee

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Title sample (if no prior publications):

Repeated Administration of D-Amphetamine Induces Distinct Alterations in Behavior and Metabolite Levels in 129Sv and Bl6 Mouse Strains

Supervisior(s): Prof MD PhD {first and family name}

Keywords: {insert keywords for match-making}

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Abstract sample (if you have prior publications; you can copy from publisher):

Repeated Administration of D-Amphetamine Induces Distinct Alterations in Behavior and Metabolite Levels in 129Sv and Bl6 Mouse Strains

Taavi Vanaveski 1,2,* Jane Narvik 1,2, Jürgen Innos 1,2, Mari-Anne Philips 1,2, Aigar Ottas 2,3, Mario Plaas 2,4, Liina Haring 2,5, Mihkel Zilmer 2,3 and Eero Vasar 1,2

1Department of Physiology, Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia
2Center of Excellence for Genomics and Translational Medicine, University of Tartu, Tartu, Estonia
3Department of Biochemistry, Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia
4Psychiatry Clinic and Center of Excellence for Genomics and Translational Medicine, Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia
5Psychiatry Clinic, Tartu University Hospital, Tartu, Estonia

Abstract
The main goal of the study was to characterize the behavioral and metabolomic profiles of repeated administration (for 11 days) of d-amphetamine (AMPH, 3 mg/kg i. p.), an indirect agonist of dopamine (DA), in widely used 129S6/SvEvTac (129Sv) and C57BL/6NTac (Bl6) mouse strains. Acute administration of AMPH (acute AMPH) induced significantly stronger motor stimulation in Bl6. However, repeated administration of AMPH (repeated AMPH) caused stronger motor sensitization in 129Sv compared acute AMPH. Bodyweight of 129Sv was reduced after repeated saline and AMPH, whereas no change occurred in Bl6. In the metabolomic study, acute AMPH induced an elevation of isoleucine and leucine, branched-chain amino acids (BCAA), whereas the level of hexoses was reduced in Bl6. Both BCAAs and hexoses remained on the level of acute AMPH after repeated AMPH in Bl6. Three biogenic amines [asymmetric dimethylarginine (ADMA), alpha-aminoadipic acid (alpha-AAA), kynurenine] were significantly reduced after repeated AMPH. Acute AMPH caused in 129Sv a significant reduction of valine, lysophosphatidylcholines (lysoPC a C16:0, lysoPC a C18:2, lysoPC a C20:4), phosphatidylcholine (PC) diacyls (PC aa C34:2, PC aa C36:2, PC aa C36:3, PC aa C36:4) and alkyl-acyls (PC ae C38:4, PC ae C40:4). However, repeated AMPH increased the levels of valine and isoleucine, long-chain acylcarnitines (C14, C14:1-OH, C16, C18:1), PC diacyls (PC aa C38:4, PC aa C38:6, PC aa C42:6), PC acyl-alkyls (PC ae C38:4, PC ae C40:4, PC ae C40:5, PC ae C40:6, PC ae C42:1, PC ae C42:3) and sphingolipids [SM(OH)C22:1, SM C24:0] compared to acute AMPH in 129Sv. Hexoses and kynurenine were reduced after repeated AMPH compared to saline in 129Sv. The established changes probably reflect a shift in energy metabolism toward lipid molecules in 129Sv because of the reduced level of hexoses. Pooled data from both strains showed that the elevation of isoleucine and leucine was a prominent biomarker of AMPH-induced behavioral sensitization. Simultaneously a significant decline of hexoses, citrulline, ADMA, and kynurenine occurred. The reduced levels of kynurenine, ADMA, and citrulline likely reflect the altered function of N-methyl-D-aspartate (NMDA) and NO systems caused by repeated AMPH. Altogether, 129Sv strain displays stronger sensitization toward AMPH and larger variance in metabolite levels than Bl6.

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