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Histology

How to calculate the total magnification of a light microscope? *

1 point

By multiplying the magnifying power of the ocular and objective lenses

By dividing the magnifying power of the ocular and objective lenses.

By multiplying the resolving power of the ocular and objective lenses.

By multiplying the magnifying power of the ocular lens by 10.

The part of bright-field microscope that collects and focuses a cone of light that illuminates the tissue slide on the stage is called: *

1 point

objective lens

condenser

ocular lens

a and c

Phase-Contrast Microscope creates contrast by: *

1 point

Changing of light speed through a specimen with different refractive indices

Staining

Using a small point of high intensive light

the amount of radiolabel applied to the specimen

In fluorescence microscopy, which of the following compounds would be useful to differentiate a DNA molecule from an RNA molecule? *

1 point

acridine orange

phalloidin

DAPI

B and C

Which microscope would be particularly useful for looking at living cells? *

1 point

Simple microscope

Compound microscope

Phase contrast microscope

Transmission electron microscope

If the surface of the specimen is dried and spray-coated with a very thin layer of heavy metal (often gold) then we expect which of the following microscopes to be used? *

1 point

Brightfield Microscope

TEM

SEM

all of the above

For what extent of resolution does TEM imaging permit? *

1 point

3*10^-8

0.3*10^-8

3*10^-6

0.3*10^-6

The maximal resolving power of the light microscope is approximately 0.2 μm, which can permit clear images magnified ……… times? *

1 point

500 - 1000

1000 – 1500

2000 – 2500

2500 -- 3000

Which of the following procedures relies on the cationic and anionic properties of the material to be stained *

1 point

Enzyme Histochemistry

Hematoxylin , eosin staining

immunohistochemistry

Metal impregnation techniques

During the preparation of a routine H&E slide, what step occurs after the tissue is preserved? *

1 point

Fixation

Embedding in paraffin

Staining

Dehydration

note: preservation=fixation

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