Amino Labs' intro to Agarose Gel Electrophoresis quiz

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After you have gone through the Agarose Gel Electrophoresis presentation, test your knowledge with this quiz!

What are the applications of agarose gel electrophoresis? Select all that apply. *

1 point

Determining the concentration of DNA

Determining DNA fragment size

Determining the orientation of DNA

Purifying DNA

Required

Which of the following statements accurately describes how DNA molecules move in an agarose gel? *

1 point

The negatively charged phosphate groups attached to only cytosine and guanine bases are pulled through the gel towards the cathodic electrode.

Current flowing from the anode to the cathode forms a slow current that pushes the DNA fragments towards the cathode.

The negatively charged phosphate backbone is pulled through the gel towards the cathode when a current is applied.

The negatively charged phosphate backbone is pulled through the gel towards the anode as the current is applied.

A sample with three DNA fragments of different sizes is placed into a singular well in an agarose gel. The fragments sizes are 300 base pair's (bp), 1000bp's, and 3000bp's. Which of the following descriptions best describes the movement of the DNA fragments once a current is applied? *

1 point

The 3000bp fragment will migrate to the cathode the fastest, followed by the 1000bp fragment and finally the 300bp fragment.

The 3000bp fragment will migrate towards the anode, the 1000bp fragment will follow and the 300bp have moved the least.

The 3000bp fragment will be closest to the anode, the 1000bp fragment will be in the middle and the 300bp fragment will be closest to the cathode

The 3000bp fragment is too large to migrate through an agarose gel. Only the 1000bp and 300bp fragments have moved. The 300bp fragment will be closest to the cathode.

Which of the following are needed to set up an agarose gel electrophoresis experiment? *

1 point

A tank with alternate electrodes on either side

A buffer containing electrolytes, nucleotides, polymerases, and 1X TAE

DNA Samples including a ladder and dye

A solidified agarose gel with wells made by a comb

Buffer containing ions

Required

The image below is an agarose gel with a ladder on the left side. If you were asked to interpret the results and estimate the size of the bands to the right, which of the following would be correct? *

1 point

750 nucleotides

850 nucleotides

850 base pairs

750 base pairs

What is the function of a 1kB ladder? *

1 point

The 1kB ladder contains an assortment of DNA fragments of different sizes that can be used as a reference point for the DNA samples.

The 1kB ladder acts as a reference point for proteins of different sizes.

The 1kB ladder contains DNA fragments that migrate towards the anode and act as a reference point for other DNA samples.

The 1kB ladder contains DNA fragments of different sizes that form tunnels in the gel matrix for the DNA samples to follow.

The brightness of the bands is related to what property of the DNA sample? *

1 point

The concentration of DNA fragments

The length of the DNA fragments

The concentration of cytosine and guanine in the DNA fragments

The concentration of RNA fragments

Which of the following options best describes the order of events that would occur in an agarose gel electrophoresis experiment. *

1 point

Place gel in the tray with wells closest to the cathode, fill the tray with buffer, add DNA ladder and samples, apply current, visualize under UV light

Place gel in the tray with wells closest to the anode, fill the tray with buffer, apply current, add DNA samples and visualize under UV light

Place gel in the tray with wells closest to the anode, fill the tray with buffer, add DNA ladder and samples, apply current, visualize under UV light.

Place gel in the tray with wells closest to the cathode, fill the tray with water, add DNA ladder and samples, apply current, visualize under UV light

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