Registration form for the second RECYCLES workshop "Metagenomics and metabarcoding approaches to describe ecological systems and infer their development", organized by Università di Pisa with the collaboration of all the RECYCLES project partners. The workshop sessions will be in hybrid mode, both in person and online through MS Teams. The location of the sessions will be Polo Didattico San Rossore, Pisa, Aula SRD. At the end of the registration form, please indicate which mode are you going to use to attend the workshop.

5, 6 and 7 of July 2022 - Università di Pisa

Session 1 - 05/07/2022 - 12:00 to 14:30 CEST. Polo Didattico San Rossore, Pisa. Aula SRD and online (Hybrid session)
Metagenomics and metabarcoding approaches to describe ecological systems and infer their development.

12:00 – Welcome to participants by Ms Eleftheria Lykouressi, Project Officer from the European Commission
12:10 – Introduction to the project and to the workshop, welcome to participants by Giulio Petroni and David Gabriel.
12:30 – Introduction to metagenomics and metabarcoding approaches: Potentialities and pitfalls (Dr. Leandro Gammuto from Università di Pisa, Italy)
13:00 – From samples to sequences - From sequences to ecological insight with examples (Dr. Francesco Vitali from Research Centre for Agriculture and Environment, Council for Agricultural Research and Economics (CREA-AA), Florence, Italy).
13:30 – The predictive functional metagenomic approach in the process studies (Dr. Simone Becarelli from Università di Pisa, Italy).

Q&A


Session 2 - 06/07/2022 - 12:00 to 14:00 CEST and 16:00 to 18:00 CEST. Polo Didattico San Rossore, Pisa. Aula SRD and online (Hybrid session)
The experiences of RECYCLES partners.

12:00 – Enhanced biogas production from sulfate rich wastewater generated from concentrated latex factory (Dr. Prawit Kongjan from Prince of Songkla University, Thailand)
12:20 – Expanding the range of application of biological process for gas and wastewater treatment (Dr. Albert Bartrolí from AERIS Tecnologías Ambientales S.L., Spain)
12:40 – Microbial diversity monitoring in an anaerobic digestor treating tannery fleshing (Dr. Gabriele D’Elia from Italprogetti Spa, Italy, and Dr. Cecilia Polizzi from Università degli Studi di Firenze, Italy)
13:00 – Microbial diversity dynamics in a UASB bioreactor targeting sulfate reduction (Prof. David Gabriel from Universitat Autònoma de Barcelona, Spain)
13:20 – Primers relevance in metabarcoding: An example for anammox reactors (Prof. Matteo Ramazzotti from Università degli Studi di Firenze, Italy)

Q&A

16:00 – Fabrication of formate dehydrogenase biocathodes for bioelectrochemical CO2 conversion (Dr. Carmina Ottone from Pontificia Universidad Católica de Valparaíso, Chile)
16:20 – Metagenomics analysis of the resistome and phageome in wastewater treatment plants in Winnipeg, Canada (Kadir Yanac, PhD student from University of Manitoba, Canada)
16:40 – Selected example of small metagenomic project: Ciliates and their bacterial symbionts/consortia (Dr. Leandro Gammuto from Università di Pisa, Italy).
17:00 – Metabarcoding analysis of storage conditions for long term experiments (Leandro Di Gloria, PhD student from Università degli Studi di Firenze, Italy).
17:20 – The mycoremediation for the treatment of the dredged sediments (Giacomo Bernabei, PhD student from Università di Pisa, Italy).
17:40 – Study of microbial communities associated with cultures of marine copepods involved in the degradation of biopolymers (Luca Niccolini from Università di Pisa, Italy).

Q&A


Session 3 - 07/07/2022 - 12:00 to 16:00 CEST. Polo Didattico San Rossore, Pisa. Aula SRD and online (Hybrid session)
Online practical on metabarcoding analysis by Dr. Francesco Vitali from the Research Centre for Agriculture and Environment, Council for Agricultural Research and Economics (CREA-AA), Florence, Italy
- Introduction: different possible approaches, tools, and definitions.
- The fundamental elements of microbial ecological analysis: metadata, otu table, taxonomy table. Importance of rich metadata annotation.
- Setting up of working environment.
- Sequence manipulation: pre-treatment.
- Sequence analysis: obtaining otu table and taxonomy table (QIIME2, all R approach eventually).



FREE registration to all sessions
Sessions hosted in Microsoft Teams (for speakers, it is necessary to download the Microsoft Teams software from Microsoft website, for audience the workshop can be followed via website).
Link to enter the workshop server will be sent by the 4th of July (check spam folder)
The entire workshop will be in English.
If interested in attending the "Online practical on metabarcoding analysis" session, please fill the questionnaire you will find at the link provided at the end of the registration form (answers will be used to have an idea of the average bioinformatic expertise of the participants).