This is the last question in this diagnostic. Most summers since 1893, young developmental and evolutionary biologists have flocked to Woods Hole, Massachusetts, to master the tricks of their trade. At the world-famous Marine Biological Laboratory there, students in its annual embryology course dissect sea urchins and comb jellies, and graft cells together from different animals. But for the last three years, the keen apprentices have been learning something new: gene editing.The precise, efficient CRISPR–Cas9 gene-editing technique has already taken life-sciences labs by storm. Now it is sweeping through evo-devo, the field that seeks to explain the developmental changes underlying evolutionary adaptations.Rather than simply infer what caused historic transitions, such as how fish developed limbs, scientists can check their hypotheses directly with CRISPR. The idea is simple: cut out the fish genes thought to be involved in making fins, and see whether the fish start to form something resembling feet. That is exactly what researchers report today in Nature, using CRISPR to help explain how fish developed feet and started walking1. Others have wielded the technique to determine how butterflies evolved exquisite colour vision, and how crustaceans acquired claws.“CRISPR is a revolution all across biology, but for evo-devo it’s transformative,” says Arnaud Martin, an evolutionary developmental biologist at George Washington University in Washington DC. “We can do things we were not able to do before.” How fins became feetNeil Shubin, a palaeontologist and developmental biologist at the University of Chicago in Illinois, has used gene-editing to examine how the tips of fish fins, or rays, were replaced by feet and digits in four-legged land vertebrates, or tetrapods.While researchers know that ancient fish developed limbs – Shubin led the team that in 2004 discovered a 375-million-year-old fossil that seemed to catch that transition in the act – they also thought that the foot was an evolutionary novelty without an equivalent in fish, because rays and feet are made of different kinds of bone.But Shubin says gene-editing has changed his mind. His team used CRISPR to engineer zebrafish lacking various combinations of the several hox13 genes they possess – genes that researchers already thought played an important role in laying down fin rays.None of the mutants grew fully fledged feet, Shubin notes, but some possessed “fingery fins” made of the same kind of bone that builds fingers and toes in tetrapods. “As a palaeontologist I studied and trained thinking these are two different kinds of bones that are completely unrelated developmentally or evolutionarily,” says Shubin. “These results challenge that assumption.”The zebrafish is a popular model organism, whose genome is regularly manipulated in the lab. But CRISPR vastly sped up the experiments performed by Shubin's team. One next step will be to knock out hox13 genes in fish species that more closely resemble the ancient fish that gained limbs, say Aditya Saxena and Kimberly Cooper, evolutionary developmental biologists at the University of California, San Diego. Those experiments are now conceivable thanks to CRISPR, they note in a commentary that accompanies Shubin's article[2]. What does the technique CRISPR do and will it apply to other unrelated species? *