Dr. Virginia Puente is a neuroscientist whose work focuses on how the structure and dynamics of the brain’s extracellular space (ECS) modulate neuronal signaling. Her research integrates advanced tools including optogenetics, fluorescence imaging, and super-resolution techniques like STED microscopy and SUSHI (Super-resolution Shadow Imaging) to examine neuronal excitability and synaptic plasticity. Dr. Puente has developed real-time tools to control and visualize proteins critical to neuronal function, such as AMPA receptors, PSD95, and Stargazin. In her work with organotypic hippocampal slice cultures, she maps ECS architecture and investigates neuronal activity, with projects supported by European and national research funding.

In this webinar, Dr. Puente will:

- Demonstrate the step-by-step process of preparing organotypic slices, including tissue sectioning and handling, to ensure viability and reproducibility

- Explain the setup and operational principles of the roller drum culture system, highlighting its role in maintaining tissue health over extended periods

- Showcase the shadow imaging technique for visualizing organotypic slices, emphasizing its advantages, technical considerations, and image interpretation

- Discuss common challenges and troubleshooting strategies in organotypic slice preparation, culture, and imaging to optimize experimental outcomes