Prerequisites
Some background in chemistry is required for this course, at least up to undergraduate
analytical chemistry. Organic chemistry and instrumental analysis are also recommended
but not required.

Class Size
Up to 10 students can participate in this course. Projects may be done in groups depending
on class size.
Evaluation
There is no formal evaluation for this IAP. Feedback and guidance will be given by the
instructor for method presentations.
Project
Pick something you’d like to separate and qualitatively or quantitatively analyze (nothing
illegal or DEA scheduled). Throughout the course, you will develop a validated method that
strives to satisfy regulatory analytical metrics (FDA, EPA, etc.), which will be discussed
in class. Results will be presented and reviewed in the last class. Projects in the past have
included:
• Capsaicin content in different pepper varieties
• Flavonoid quantitation in tea leaves
• Residual tetracycline antibiotics in dairy products

Reading and Research
In the interest of time, we will be using a simulator spreadsheet developed by Davy Guillarme
et al. at University of Geneva. The simulator can be downloaded for free at:
https://ispso.unige.ch/labs/fanal/telechargement-practical hplc simulator v1.0-22?macro=no
At the end of the week, we will be working in the lab to gain hands-on experience with an
HPLC. Your method’s success will greatly depend on your understanding of the underlying
theories and simulations practiced beforehand.
Recommended supplemental books that are not expected to be completed within one
week:
• Pitfalls and Errors of HPLC in Pictures by Veronika R. Meyer
• High Performence Gradient Elution by Lloyd Snyder and John Dolan
• An Introduction to Separation Science by Lloyd Snyder and Barry Karger
• High Pressure Liquid Chromatography by Phyllis Brown