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Virus Injection Protocols in Ferret
Notes of past experiences with virus injection protocols for ferret. Note that this just describes procedures for virus delivery, and there is a second table for viruses, titers, etc.
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Email
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Injection Protocol Name
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(a name you provide for reference, e.g., "Briggs LGN 1" or "Smith 1")
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Target
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(e.g., "cortical surface", "thalamus")
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Injection tool
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Drummond Scientific Nanoject II
Hamilton Syringe
WPI Nano Injector
Other:
Typical number of injection penetrations
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Total number of injection sites per penetration
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Depth of injection site(s) for each penetration
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Total volume of injection at each injection site (nl)
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Number of injections at each injection site
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Volume of each (small) injection at each injection site (nl)
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Do you perform any special treatment of the skull, dura, or brain prior to injection?
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How do you seal the craniotomy after the injection?
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Outcome
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Worked well, paper published
Working well right now, feeling optimistic
Not successful with medium effort
Not successful with substantial effort
Other:
References (please include PubMed links)
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If your procedures have been used in published reports, list those here.
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Comments
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