ABE MA 2021 - 2022 Scheduling Survey

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This form will allow the ABE MA staff to determine what your needs are and how to support you during this program year. Both returning and new teachers must fill out this form by 5pm on Friday, August 20th.

NB: The ABE MA Team must comply with changing regulations and policies set by our host institutions (Harvard, STCC, Essex Tech, and the MBL).

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Teacher First Name *

Teacher Last Name *

Email *

Which email is best to share Google Drive documents with?

School Name *

School District *

Is this your first year doing ABE? *

Yes

Is this your school’s first year doing ABE? *

Yes

Which site will you participate out of? *

Harvard University, Cambridge

The MBL, Woods Hole

Springfield Technical Community College

Essex Tech (New Pilot Distribution Site)

Pflichtfrage

Partnership Type

Schools can either be reagent partners or partial/full kit partners. This year, we are supporting a "demo" version (see below).

Typical Partnership Type *

Reagent Partner: I have all the equipment, I just need reagents!

Kit Partner: I need everything!

Partial Kit Partner: I have some equipment but need to borrow as well!

Partnership Type *

Reagent Partner (Class): I only need reagents for classroom use with my students

Reagent Partner (Demo): I only need reagents for myself to demo the labs for my students

Partial Kit Partner (Class): I have some equipment but need to borrow additional items for classroom use with my students (THIS MIGHT NOT BE SUPPORTED)

Partial Kit Partner (Demo): I have some equipment but need to borrow additional items to demo the labs for my students

Full Kit (Class): I need everything for classroom use with my students

Full Kit (Demo): I need all equipment for one demo set.

Lab Selection

Which labs do you currently plan on implementing into the 2020-2021 academic year? *

For labs 2 and 4, please indicate if you will be doing the 2a/4a/5a combination or the 2/3/4/5 (ligation) combination. Additionally, if you are interested in just doing the bacterial transformation and not labs 2/2a or 4/4a, please select Lab 5b, which is a stand alone.

Lab 1: Intro to Micropipettes and Gel Electrophoresis

Lab 2: Digesting the pKAN-R and pARA

Lab 2a: Digesting the pARA-R Plasmid

Lab 3: Building the pARA-R Plasmid

Lab 4: Verification of Restriction and Ligation using Gel Electrophoresis

Lab 4a: Verification of the Recombinant Plasmid using Gel Electrophoresis

Lab 5: Transforming Bacteria with the Ligation Products

Lab 5a: Transforming Bacteria with the pARA-R Plasmid

Lab 5b: Transforming Bacteria with a Recombinant Plasmid (stand alone lab)

Lab 6: Purifying the Fluorescent Protein

PCR: ABE Colony PCR Lab

PCR: GMO Lab

PCR: PTC (Please check with your administration if DNA collection is allowed. We will supply you with a consent form to share with students.)

PCR: mtDNA (Please check with your administration if DNA collection is allowed. We will supply you with a consent form to share with students.)

PCR: DS180 (Please check with your administration if DNA collection is allowed. We will supply you with a consent form to share with students.)

Pflichtfrage

Student Numbers

Please list how many students will participate in each lab. Enter 0 (zero) if you do not plan on doing a select lab.

If you plan to demo the lab for your students, please write in "Demo" to signify you need one set.

Lab 1 *

Lab 2 *

Lab 2a *

Lab 3 *

Lab 4 *

Lab 4a *

Lab 5 *

Lab 5a *

Lab 5b *

Lab 6 *

PCR: ABE Colony PCR Lab *

PCR: GMO Lab *

PCR: PTC *

PCR: mtDNA *

PCR: DS180 *

Class and Course Information

Total estimated number of students using ABE (all classes) *

Number of Classes using ABE *

The number of classes and/or sections doing ABE with this teacher. For example, if a teacher is doing ABE with two Introductory Biology classes and one Advanced Biology class, the number of classes would be 3.

Number of Courses using ABE *

The number of courses this ABE is using ABE in. For example, if a teacher is doing ABE with two Introductory Biology classes and one Advanced Biology class, the number of courses would be 2. If the teacher is using ABE in more than one course, please provide the course details (below) for each course.

What course type will you implement ABE in? *

Middle School

Introductory Biology

Advanced/Honors Biology

AP Biology

Biotechnology

Forensics

Marine Biology

University Level

Sonstiges:

Pflichtfrage

Biotechnology Equipment Needed

Instead of asking for your inventory, we're just looking to know what pieces of equipment you may need to implement the ABE labs.

Biotechnology Equipment Needed *

Gel electrophoresis boxes

Gel imaging system (blue light box)

P-20 pipettes

P-200 pipettes

P-1000 pipettes

Incubator

Water bath

Platform shaker

Centrifuge

PCR machine

Chromatography columns

Sonstiges:

Pflichtfrage

Scheduling

Please select your top THREE preferred cycles. Keep in mind that each cycle houses only a limited number of slots. If you are a reagent partner, you will have more flexibility with scheduling.

First Choice Cycle *

Cycle 1: 9/27 - 10/22

Cycle 2: 11/1 - 11/29

Cycle 3: 12/6 - 12/22

Cycle 4: 1/10 - 1/28

Cycle 5: 2/7 - 3/4

Cycle 6: 3/14 - 4/8

Cycle 7: 4/18 - 5/13

Cycle 8: 5/23 - 6/22

Second Choice Cycle *

Cycle 1: 9/27 - 10/22

Cycle 2: 11/1 - 11/29

Cycle 3: 12/6 - 12/22

Cycle 4: 1/10 - 1/28

Cycle 5: 2/7 - 3/4

Cycle 6: 3/14 - 4/8

Cycle 7: 4/18 - 5/13

Cycle 8: 5/23 - 6/22

Third Choice Cycle *

Cycle 1: 9/27 - 10/22

Cycle 2: 11/1 - 11/29

Cycle 3: 12/6 - 12/22

Cycle 4: 1/10 - 1/28

Cycle 5: 2/7 - 3/4

Cycle 6: 3/14 - 4/8

Cycle 7: 4/18 - 5/13

Cycle 8: 5/23 - 6/22

Any other information that the ABE MA Team should know?

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